原创《基于SELDI—TOF—MS冷却滩羊肉微生物差异蛋白分析》：本论文主要论述了羊肉论文范文相关的参考文献,对您的论文写作有参考作用。
摘 要：以托盘密封包装的冷鲜滩羊肉为研究对象,基于组合蛋白芯片与表面增强激光解吸电离飞行时间质谱（surface enhanced laser desorption ionization time-of-flight mass spectrometry,SELDI-TOF-MS）平台对滩羊肉表面微生物蛋白进行分析,区分不同贮藏时间生物样本之间的差异.通过正交矫正偏最小二乘法判别分析判断模型第一主成分的得分值（VIP阈值>1）,并结合学生氏t检验的P值（阈值<0.05）寻找差异性表达蛋白.结果表明：应用Biomarker Wizard软件分析找出差异蛋白峰132 个,其中75 个差异蛋白峰可根据质荷比经Swiss-Prot数据库检索出对应的蛋白质,其余57 个差异蛋白峰对应的为未知蛋白；从上述差异蛋白中未找到酶类的差异蛋白,但是找到了可能和滩羊肉菌体有关联的19 个菌体蛋白.SELDI-TOF-MS技术能够用于检测冷鲜滩羊肉不同贮藏阶段基因表达的各种蛋白质,从而发现大量具有价值的蛋白质标志分子.
关键词：滩羊肉；微生物；表面增强激光解吸电离飞行时间质谱；差异蛋白
Abstract： Microbial proteins on the surface of tray-packaged chilled Tan sheep meat were analyzed by surface enhanced laser desorption ionization time-of-flight mass spectrometry （SELDI-TOF-MS） and biological samples collected at different times during chilled storage were differentiated. The variable importance in the projection （VIP） value （threshold > 1） of the first principal component of the orthogonal partial least squares-discriminant analysis （OPLS-DA） model was determined and combined with the P value （threshold < 0.05） of student’s t test to find out the differentially expressed proteins. The results showed that 132 differential protein peaks were obtained by Biomarker Wizard software, 75 of which were identified by searching in the Swiss-Prot database based on their mass-to-charge ratio, and the remaining 57 were unknown proteins. These differential proteins did not include enzymes but included 19 proteins that might associated with bacteria on mutton meat. In summary, SELDI-TOF-MS allows the detection of various differentially expressed microbial proteins at different storage times of chilled Tan sheep meat and consequently the identification of a large number of valuable protein markers.
Keywords： chilled Tan sheep meat； microorganism； surface enhanced laser desorption ionization time-of-flight mass spectrometry （SELDI-TOF-MS）； differential protein
DOI：10.7506/rlyj1001-8123-201802006
中图分类号：TS251.1 文献标志码：A文章编号：1001-8123（2018）02-0033-07
引文格式：
胡倩倩, 杨波, 罗瑞明, 等. 基于SELDI-TOF-MS的冷却滩羊肉微生物差异蛋白分析[J]. 肉类研究, 2018, 32（2）： 33-39. DOI：10.7506/rlyj1001-8123-201802006. http：//www.rlyj.pub
HU Qianqian, YANG Bo, LUO Ruiming, et al. Surface enhanced laser desorption ionization time-of-flight mass spectrometry analysis of surface microbial differential proteins in chilled tan sheep meat[J]. Meat Research, 2018, 32（2）： 33-39. DOI：10.7506/rlyj1001-8123-201802006. http：//www.rlyj.pub
寧夏盐池滩羊是经长期自然选择和人工选育而成的裘肉兼用型品种,被农业部列为国家二级保护品种.滩羊肉脂肪分布均匀、膻味较轻、肉质细嫩鲜美、营养丰富、风味独特,深受广大消费者的喜爱[1-3].冷鲜滩羊肉是指对严格按照检疫制度屠宰后的*迅速进行冷却处理,使*温度（以后腿为测量点）在24 h内降为0～4 ℃,并在后续的流通过程中始终保持在0～4 ℃范围内的鲜羊肉[4-8].
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结论：基于SELDI—TOF—MS冷却滩羊肉微生物差异蛋白分析为关于羊肉方面的论文题目、论文提纲、羊肉的功效与作用论文开题报告、文献综述、参考文献的相关大学硕士和本科毕业论文。
