原创《强骨宝对去势大鼠原发性膝骨关节炎关节软骨影响实验》：这篇去势论文范文为免费优秀学术论文范文,可用于相关写作参考。
【摘 要】目的：建立去势后原发性膝骨关节炎大鼠模型,观察强骨宝对模型鼠关节软骨形态学及软骨基质的影响,为进一步研究提供基础.方法：①去势后原发性膝骨关节炎模型建立：麻醉下摘除双侧卵巢1周后行疲劳性跑步训练,跑台坡度-15°,频率28 m·min-1,60 min·d-1,每周6 d,建立膝骨关节炎模型.分别于2,4,6周后检测尿液中Ⅱ型胶原羧基端肽（CTX-Ⅱ）,制作股骨髁病理切片鉴定模型.②动物分组和给药：将造模成功的大鼠随机分为模型对照组、强骨宝组、阳性对照组,分别给予相应处理.③取材和检测：给药4周后,取尿液、血清、双侧股骨髁,检测尿液中CTX-Ⅱ含量,股骨髁软骨经HE染色处理后行Mankin评分.结果：①模型鉴定：根据尿液CTX-Ⅱ含量及软骨Mankin评分结果,造模
4周后关节软骨开始退变,6周后退变明显,模型成功.②给药治疗：Mankin评分及CTX-Ⅱ含量测定结果,和空白对照组比较,模型对照组、强骨宝组、阳性对照组均增高（P < 0.01或P < 0.05）；与模型对照组比较,强骨宝组、阳性对照组均降低（P < 0.05）；与阳性对照组比较,强骨宝组差异无统计学意义（P > 0.05）.结论：强骨宝可减缓原发性膝骨关节炎模型鼠的软骨损伤进程.
【关键词】 骨关节炎,膝；强骨宝；动物模型；大鼠；实验研究
doi：10.3969/j.issn.2095-4174.2016.04.002
Experimental Study on the Effect of Qianggu Bao （强骨宝） on the Articular Cartilage of Primary Knee Osteoarthritis in Ovariectomized Rats
WANG Wen-sheng,CHEN Wei,SHEN Bao-lei,XU Bo,ZHANG Li
【ABSTRACT】Objective：To observe the effect of Qianggu Bao（强骨宝）on the articular cartilage of primary
knee osteoarthritis in ovariectomized rats.Methods：①Establishing models of ovariectomized rats with primary knee osteoarthritis：After anesthesia,bilateral ovariectomy was performed for the experimental rats.After one week,they were forced to do fatigued running training on 15-degree treadmill with frequency of 28 m·min-1,60 min·d-1,for 6 days a week to establish knee osteoarthritis models.CTX-Ⅱin urine was detected respectively after 2,4 and 6 weeks,making pathological identification model of femoral condyle.②Grouping and administration：Successfully modeled rats were randomly divided into a model group,a Qianggu Bao group and a positive control group,and each group was given corresponding treatment.③Sanjmpling and detection：After 4 weeks of administration,urine,serum and bilateral femoral condyle were sampled to detect the content of CTX-Ⅱin urine and make Mankin scores to femoral condyle cartilage after HE staining.Results：①Model identification：According to the content of
CTX-Ⅱin urine and Mankin scores of femoral condyle cartilage,articular cartilage degeneration began after 4 weeks of modeling,which was obvious after 6 weeks,so the models were successfully established.
② Administration for treatment：Compared with the blank control group,Mankin score and CTX-Ⅱcontent of the model control group,the Qianggu Bao group and the positive control group all increased （P < 0.01 or P < 0.05）；compared with the model control group,those of the Qianggu Bao group and the positive control group decreased （P < 0.05）；and the difference between the positive control group and the Qianggu Bao group had no statistical significance （P > 0.05）.Conclusion：Qianggu Bao can slow down the process of cartilage damage in model rats with primary knee osteoarthritis.
去势论文参考资料：
结论：强骨宝对去势大鼠原发性膝骨关节炎关节软骨影响实验为适合不知如何写去势方面的相关专业大学硕士和本科毕业论文以及关于去势抵抗性前列腺癌论文开题报告范文和相关职称论文写作参考文献资料下载。
